WinMDI is an older 16-bit Windows application and reads most FCS 2.0 compliant files known from most cytometer manufacturers at the time it was last compiled (in 2000), and therefore does not recognize FCS 3.0 BD digital data or other data where the raw values are are either stored at greater than 16 bits as signed/unsigned integers, or as 32 bit single precision floats (where $datatype = F). It is possible to export BD FCS 3.0 data as FCS 2.0 from within DiVa, which decreases the resolution 256-fold and does not add any baseline offsets to emulate analog baseline logrithmic amplifier behavior (i.e. zero is really zero and very dim events will be accrued on axis). You can do this, but it is not recommended to export BD DiVa FCS 3.0 files as FCS 2.0 for analysis with WinMDI.
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